CHAPTER 4: Class 3 Enzymes (Hydrolases)

This enzyme is mainly found in the prostate and comprises about one-third to one-half of the blood.

• ACP

• ALP

• Lipase

• Amylase

Acid phosphatase is a labile enzyme.

• True

• False

This method produces a yellow color when measuring the activity of ACP.

• p-Nitrophenylphosphate

• Roy Method

• 4-Nitrophenylphosphate

• Bowers-McComb

All are assays of isoenzymes of ACP, except:

• Chemical method

• Electrophoresis

• Immunoassay

• Immunologic method

All the following are related to prostatic carcinoma, except:

• This disease is common among men over the age of 50 years old.

• Major diagnostic significance particularly in metastatic carcinoma of the prostate.

• Stage III or C have a survival rate of 70-90%.

• Prostatic specific antigen is a useful tool for screening and diagnosis for prostate cancer.

All are activators of alkaline phosphatase, except:

• Magnesium

• Cobalt

• Manganese

• Zinc

The slowest moving isoenzyme in ALP.

• Liver

• Bone

• Placenta

• Intestine

ALP must be evaluated immediately because activity in the serum decreases due to the gradual development of a more acidic pH because CO₂ is lost.

• True

• False

The most useful single technique for ALP isoenzyme analysis.

• Use of heat stability

• Use of inhibitors

• Electrophoresis

• Immunologic method

These are diseases where levels of ALP are elevated, except:

• Scurvy

• Paget’s disease

• Hyperthyroidism

• Diabetes mellitus