Protoplast: Introduction & Isolation

Protoplasts are__________

• Cell without a nucleus

• Cell without a Cell wall

• Cell without a Plasma membrane

• Cell without a genetic material

__________ is the first step for protoplast culture

• Selection of Explant

• Viability testing for protoplast

• Isolation of protoplast

• Preparation of culture media

Cellulase enzyme in isolation of protoplast is used ________

• To degrade proteins

• To degrade cellulose

• To degrade pectin

• To degrade hemicellulose

The production of hybrid plants through fusion of two different somatic protoplasts is known as

• Protoplast hybrids

• Protoplast fusion

• Somatic hybridization

• Somatic hybrids

1st report on protoplast fusion-derived somatic hybrid plant was reported by

• Carlson et al

• Hanstein

• Klercker

• Cocking

The word “PROTOPLAST” was coined by

• Hanstein

• Klercker

• Cocking

• Tkebe et al

Isolation of protoplasts mechanically from higher plants was pioneered by

• Klercher

• Tkebe et al

• Cocking

• Both A & B

The principle deficiency of mechanical method of isolation is _________

• protoplasts released are few in number.

• protoplasts are damaged

• Viability of protoplasts is high

• All of the above

The most commonly used pectinase is

• Macerozyme

• Rhozyme HP 150

• Cellulase

• Pectiolose

Friable tissues are having _________

• low starch content generally yields better results

• high starch content generally yields better results

• low sucrose content generally yields better results

• low sucrose content generally yields better results