Histopathology Midterms Review

Process whereby the clearing agent is completely removed

• Impregnation

• Embedding

Process whereby the impregnated tissue is placed into a precisely arranged position in a mold

• Embedding

• Impregnation

4 types of impregnation and embedding medium

• Paraffin wax

  Celloidin

  Gelatin

  Plastic

• Paraplast

  Embeddol

  Ester wax

  Carbowax

Simple, most common and best embedding medium used for routine tissue processing

• Paraffin

• Xylene

Paraffin wax is solid at room temperature but its melting point is

• 65°C - 70°C

• 60°C - 65°C

Most common melting point of paraffin wax for histological use

• 56°C - 58°C

• 65°C - 70°C

Temperature to decrease viscosity and improve infiltration of the tissue

• 60°C - 65°C

• 39°C - 68°C

Paraffin wax is traditionally marketed by what melting points

• 39°C - 68°C

• 60°C - 65°C

Which common waxes is normally used for routine work

• 45°C

• 52°C

• 56°C

• 58°C

Ways by which paraffin wax impregnation and embedding may be performed

• Manual processing

• Automatic processing

• Vacuum embedding