FRET Quiz

What does the instrumentation known as FRET stand for?

• For Real Energy Transfer

• Follow Roman's Explanation Today

• Fluorescence Resonance Energy Transfer

• Fugitive Recovery Enforcement Team

FRET is a distance-dependent physical process by which energy is transferred non radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor)

• True

• False

The donor and acceptor must be in close proximity.

• True

• False

What is the distance range in FRET?

• 1-10 Angstrom

• 1-10 cm

• 10-100 Angstrom

• 10-100 nm

Does FRET require the image on the left or the right? And which is the acceptor and donor?

• Left, with blue as the acceptor, and yellow as the donor

• Right with blue as the donor and yellow as the acceptor

• Right, with blue as the acceptor, and yellow as the donor

• Left with blue as the donor and yellow as the acceptor

Which part of the instrument of FRET examines and records the cells using standard brightfield, phase contrast, or differential interference contrast (DIC) illumination?

• Arc-Discharge Lamphouse

• Ar-Kr Laser System with AOTF

• Cooled Gen III Intensified CCD Camera Systems

• Tungsten Halogen Lamphouse

Which is NOT an application of FRET?

• Protein-Protein Interactions

• Molecular Dimerization

• Mendoza's Cheese

• Ligand Binding to acceptor

Which advantage of FRET makes it easy to set up and calculate?

• Acceptor Photobleaching

• Sensitized emission

• Andre excitation

• Andre emission