Biotechnology Restriction Enzymes, Gel Electrophoresis

1. If the recognition sequence of the restriction enzyme HindIII is AAGCTT, then how many covalent bonds will be broken by the enzyme in the following DNA molecule?

• 1

• 2

• 3

• 4

Restriction Enzymes are isolated from :

• a).Protozoa

• b). Bacteria

• c).Virus

• d).Fungi

Restriction Enzymes were primarily used as :

• a).Defence by Protozoa against antibiotics

• b).Tool by Virus to cleave Bacterial genome

• c)Defence by Bacteria against bacteriophages viruses

• d).All of the above

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

Tags

NGSS.HS-LS1-1

What is this

• plasmid
• recombinant bacterium
• recombinant DNA
• sticky ends

Tags

NGSS.HS-LS3-2

An enzyme that cut DNA molecules at a specific “recognition site” is called

• Restriction enzyme

• DNA polymerase

• RNA polymerase

• Taq polymerase

What is a restriction enzyme?

• An enzyme that cuts DNA

• An enzyme that add to the DNA strand

• An enzyme that builds proteins

• An enzyme that breaks down lipids

Tags

NGSS.HS-LS3-2

The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....

• sticky end.

• base sequence.

• single strands.

• restriction fragments.

What is a palindrome?

• A sticky end resulting from a restriction enzyme

• A blunt end resulting from a restriction enzyme

• A sequence that reads the same forwards and backwards

• A sequence that is complementary to the other

Which restriction enzymes from above make blunt cuts? (2)

• Alul

• HaeIII

• BamHI

• HindIII

• EcoRI