Immunology Lab Final!

Immunology Lab Final!

University

24 Qs

quiz-placeholder

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Immunology Lab Final!

Immunology Lab Final!

Assessment

Quiz

Other

University

Medium

Created by

Hunter Leyendecker

Used 11+ times

FREE Resource

24 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

How are proteins separated on a western blot?
By molecular weight
By appearance
By amino acid composition

2.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

What is added to a well in a western blot to aid in size determination of the protein?
A protein sorter
A molecular weight ladder
Antibody markers

3.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

What is added during western blot incubation that allows us to detect a specific protein type?
A primary antibody against the desired protein type
A denaturant for the proteins
A secondary antibody for any non-specific protein
PBS tween

4.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

What is added to a western blot that allows us to visualize the bands (hint: it is one of the last steps in the method)?
A primary antibody against the specific protein type
Trypan blue dye
A secondary antibody labeled with HRP or a fluorescent molecule
A primary antibody with no label

5.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

Which of the following are types of ELISAs?
Direct
Indirect
Inverse
A&B
All of the above

6.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

What is the purpose of a standard in an ELISA?
To improve the accuracy of the ELISA
To generate a standard curve to compare our data to
For funsies :)

7.

MULTIPLE CHOICE QUESTION

45 sec • 1 pt

What is a direct ELISA?
An antibody binding to an antibody
An antibody binding to an antigen
An antibody binding to another antibody that bound to an antigen
An antibody binding to an antigen, which bound to another antibody

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