Immunology Lab Final!

How are proteins separated on a western blot?

• By molecular weight
• By appearance
• By amino acid composition

What is added to a well in a western blot to aid in size determination of the protein?

• A protein sorter
• A molecular weight ladder
• Antibody markers

What is added during western blot incubation that allows us to detect a specific protein type?

• A primary antibody against the desired protein type
• A denaturant for the proteins
• A secondary antibody for any non-specific protein
• PBS tween

What is added to a western blot that allows us to visualize the bands (hint: it is one of the last steps in the method)?

• A primary antibody against the specific protein type
• Trypan blue dye
• A secondary antibody labeled with HRP or a fluorescent molecule
• A primary antibody with no label

Which of the following are types of ELISAs?

• Direct
• Indirect
• Inverse
• A&B
• All of the above

What is the purpose of a standard in an ELISA?

• To improve the accuracy of the ELISA
• To generate a standard curve to compare our data to
• For funsies :)

What is a direct ELISA?

• An antibody binding to an antibody
• An antibody binding to an antigen
• An antibody binding to another antibody that bound to an antigen
• An antibody binding to an antigen, which bound to another antibody

What is an indirect ELISA?

• An antibody binding to an antibody
• An antibody binding to an antigen
• An antibody binding to another antibody that bound to an antigen
• An antibody binding to an antigen, which bound to another antibody

What is a sandwich/capture ELISA?

• An antibody binding to an antibody
• An antibody binding to an antigen
• An antibody binding to another antibody that bound to an antigen
• An antibody binding to an antigen, which bound to another antibody

What does propidium iodide (red fluorophore) bind to?

• Actin
• DNA
• RNA
• A & B
• B & C