CVS_imaging strategies

Pick the wrong problem / imaging method pair

• CT - lung fibrosis

• PET - tumour metastases

• Ultrasound - broken rib

• MRI - dilated cardiomyopathy

Which imaging technique relies on gamma - ray emission?

• PET

• SPECT

• CT

• MRI

Which imaging technique was used for this image

• MRI

• synchrotron µCT

• SPECT

• Ultrasound with micro-bubble contrast

You have an animal model for Alzheimer's disease, you are interested in seeing low synaptic activity regions in the brain of that animal. Which imaging technique do you use?

• NIFR optical imaging

• anatomical MRI

• CT

• PET

You would like to know if a protein is involved in upregulating a certain signalling cascade. What do you do first?

• Use a cell line with the correct background, label the protein with a fluorescent tag and do optical imaging

• Use a cell line with the correct background and knock out the corresponding gene.

• Breed a KO mouse model and image using MRI.

• Isolate the protein from mouse tissue and run shotgun proteomics.

You are investigating adipose tissue stem cell grafts to help with recovery post myocardial infarction. The cells express GFP and Luciferase genes. Which of the following techniques will help you determine the survival and migration of the graft cells?

• BLI optical imaging

• T1 MRI

• CT

• PET

You would like to develop bioptome forceps for endomyocardial biopsies which can be performed under MRI conditions. Which investigation route do you take before it is tested in a patient?

Part 1: Which material do you use for the components of the bioptome?

• Steel

• Resin

• Gold

• Titanium

You would like to develop bioptome forceps for endomyocardial biopsies which can be performed under MRI conditions. Which investigation route do you take before it is tested in a patient.

Part 2: you have built the device, which test do you perform first?

• use the bioptome under MRI in situ in a wt mouse to test the visibility of the device

• use the device ex vivo on pig heart tissue without MRI to test the cutting capability

• use the device under fluorography in vivo in a wt pig to test the manoeuvrability

• use the device under MRI in a healthy volunteer to test everything

Continued: You have obtained the biopsy, it should be of sufficient quality to tell whether a transplant has been rejected.

Part 3: what imaging technique do you use to examine the biopsy?

• phase contrast µCT imaging to examine the integrity in 3D and then histology

• ultrasound the heart of the subject to examine the biopsied spot, then do histology on the biopsy

• Image the biopsy with MRI for 3D integrity analysis

• perform PET imaging on the subject, then do a PCR on the biopsy.