Week 3

DNA polymerase has

• 5’→3’ DNA synthesis and 5’→3’ exonuclease activity
• 5’→3’ DNA synthesis and 3’→5’ exonuclease activity
• 3’→5’ DNA synthesis and 5’→3’ exonuclease activity 
• 3’→5’ DNA synthesis and 3’→5’ exonuclease activity

What is the correct order of proteins involved in DNA replication?

• SSB proteins, helicase, DNA polymerase, DNA primase, ligase
• DNA primase, helicase, DNA polymerase, SSB proteins, ligase
• DNA polymerase, helicase, DNA primase, SSB proteins, ligase
• Helicase, SSB proteins, DNA primase, DNA polymerase, ligase

What is false about DNA replication?

• Clamp assembly occurs at the 3' end of the growing chain
• The lagging strand is made in the 3’ to 5’ direction
• Discontinuous lagging strand fragments are Okazaki fragments
• DNA replication occurs bi-directionally

What is the role of MutS?

• Scans nearby DNA for a nick
• Triggers degradation of nicked strand
• Binds specifically to a mismatched base pair
• Methylates mismatched base pairs

What is the role of telomerase?

• To extend the lagging strand in a 5’ to 3’ direction
• To extend the lagging strand in a 3’ to 5’ direction
• To extend the parental strand in a 3’ to 5’ direction
• To extend the parental strand in a 5’ to 3’ direction

Which of the following correctly orders the levels of eukaryotic DNA packaging from smallest to largest?

• DNA<Chromatin<Chromosome<Nucleosome
• DNA<Chromatin<Nucleosome<Chromosome
• Nucleosome<DNA<Chromatin<Chromosome
• DNA<Nucleosome<Chromatin<Chromosome

Which of the following is true in DNA replication?

• DNA polymerase adds nucleotides to the 5’ end of growing strand
• Nucleotides are added by complementary base pairing with coding strand
• deoxynucleotide diphosphates are hydrolyzed to provide energy

• None of the above