Week 10

Which situation would increase transcription in eukaryotes most?

• SWI-SNF protein complex absent, no nudging, no acetylation

• SWI-SNF protein complex present, nudging present, no acetylation

• SWI-SNF protein complex absent, no nudging, acetylation present

• SWI-SNF protein complex present, nudging, and acetylation present

DNA methylation often occurs at the 5’ cytosine in the CG dinucleotide and is associated with inactive genes -

• True

• False

What is not a characteristic of proteasome?

• Uses ATP

• Binds multi-ubiquitinated proteins

• Produce 3-25aa long peptides.

• Has ubiquitin hydrolase

What is true regarding proteins which are directed to the ER?

• There is a nuclear localisation sequence (NLS) attached to the protein.

• SRPs bind to the signal peptide of the protein.

• Disulphide bonds can form between 2 alanine side chains

• Poly-ubiquitination occurs post-translationally

What would you expect around an active gene sequence?

• Activation of HAT

• Upregulated protein inhibitors of HAT

• Histone spreading proteins

• Activators of HDAC

What may not result from a heavily mutated Methyl cytosine binding protein?

• More free HDAC in the nucleus

• Less methylation of Cytosines in CpG

• No nucleosome aggregation

• More transcription