Microscopy Revision

The highest magnification for an electron microscope is

• 1000 x

• 10,000 x

• 100,000 x

• 1,000,000 x

Which of the following lenses on a compound light microscope DOES NOT provide magnification?

• ocular lens

• 4x objective lens

• 100x objective lens

• condenser lens

The size of a specimen at 40x objective magnification is 0.2 millimeters. What is the actual size of the specimen in micrometers?

• 0.005

• 0.05

• 0.5

• 5.0

Which of the following statements about the use of the light microscope is TRUE?

• The higher the magnification, the higher the resolution.

• The higher the magnification, the shorter the working distance.

• The higher the magnification, lesser light is required.

• The higher the magnification, the clearer the image.

Which of the following parts of the light microscope helps to adjust the aperture to control the amount of light to the specimen on the slide?

• Light switch.

• Light intensity knob.

• Condenser lens.

• Diaphragm lever.

Which of the following is TRUE about crystal violet stain?

• It is a basic dye that is negatively-charged.

• It is an acidic dye that is positively-charged.

• It is a basic dye that is positively-charged.

• It is an acidic dye that is negatively-charged.

Which of the following statements is CORRECT?

• Both Specimen A & B are positively-stained.

• Specimen A is negatively-stained while Specimen B is positively-stained.

• Both Specimen A & B are negatively-stained.

• Specimen A is positively-stained while Specimen B is negatively-stained.

Which of the following is an example of an acidic dye?

• Safranin.

• Nigrosin.

• Crystal violet.

• Methylene blue.

Identify the type of stain applied in this image.

• No stain.

• Negative stain.

• Capsule stain.

• Endospore stain.

Identify the genus of the specimen as seen under the microscope.

• Bacillus.

• Clostridium.

• Streptococcus.

• Staphylococcus.