ACC 2

Which of the following is used for high-risk pathogens?

•  Biosafety cabinet (BSC) Class I

•  Biosafety cabinet (BSC) Class II

•  Biosafety cabinet (BSC) Class III

Class II BSC keeps materials within the cabinet sterile as well as protect the scientist and the environment from biohazardous substances.

• True

• False

For BSC Class II, the air circulated within the cabinet is kept sterile by high efficiency particulate air (HEPA) filters.

• True

• False

Which of the following provides a sterile work environment. Does not provide protection to user or environment.

• biosafety cabinet

• laminar flow hood

Cell culture incubator helps to regulate.... (more than 1 answer)

• temperature

• carbon dioxide

• humidity

What is the concentration of carbon dioxide in the cell culture incubator?

• 1-5 %

• 5-10 %

• 10- 15 %

• 25-35%

Which of the following is used for large scale cell culture?

• flasks

• plates

• bioreactors

What type of cell is shown?

• suspension

• adherent

What type of cell is shown?

• suspension

• adherent

The purpose of subculturing is to ........ (more than 1 answer)

• maintain the cells at lag phase

• decrease cell density so that they is enough space

• increase the volume of culture medium

• store the cells in liquid nitrogen