Chapter 6.2, Enzymes and protein in DNA replication process

Function of helicase is to

• unwind the double helix strand of DNA

• catalyze the formation of lagging strand

• Join two okazaki fragment together

• catalyze the addition of free DNA nucleotide.

Which enzyme help relief strand caused by overwinding

• Helicase

• Topoisomerase

• DNA Polymerase

• DNA ligase

Addition of RNA primer are catalyzed by

• Single strand binding protein

• DNA ligase

• DNA primase

• DNA Polymerase

DNA Polymerase II catalyze

• the addition RNA primer

• Addition of free DNA nucleotide complementary to the template strand

• remove RNA primer and replace with DNA nucleotide

• Join Okazaki Fragment

DNA polymerase I removes

• RNA primer

• DNA nucleotide

• RNA Nucleotide

• Okazaki Fragment

joining of sugar phosphate backbone between Okazaki fragment are catalyzed by

• DNA Primase

• DNA polymerase I

• Helicase

• DNA ligase