1ST SUMMATIVE TEST IN GEN BIO II (2023-2024))

What is the main function of the ribosomes in prokaryotic cells?

• DNA replication

• Movement

• Protein synthesis

• Cell wall formation

GMO stands for ____

• Genetically Made Organism

• Genetic Manipulated Organisms

• Genetically Modified Organism

• Growing Many Organisms

It is the using of the whole microorganism without alterations of its structure or parts in the process of making a product that can help mankind.

• Genetic engineering 

• traditional biotechnology

• Modern biotechnology 

• recombinant  DNA technique

Scientists have produced a genetically modified orange tree that is resistant to disease. Which of the following is the best example of a potential benefit to society that could result from this biotechnology?

• reduction in orange crops produced

• increase in orange crops produced

• increase in the cost of orange crop

• a change in the distribution of insects that pollinate orange flowers

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• Hydrogen bond 

• Ligase

• Covalent bond 

• restriction endonuclease

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?

I) Transform bacteria with recombinant DNA molecule.

II) Extract plasmid DNA from bacterial cells.

III) Cut the plasmid DNA using restriction enzymes.

IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.

V) Use ligase to seal plasmid DNA to non-plasmid DNA

• I, II, IV, III, V

• III, II, IV, V, I

• II, III, IV, V, I

• III, IV, V, I, II

Dr. Lee is working on a project to enhance the nutritional value of rice by introducing a gene from a daffodil that produces beta-carotene. Which of the following best describes the purpose of this genetic modification?

• To increase the rice plant's resistance to pests

• To develop a rice variety with a higher yield

• To improve the rice plant's tolerance to herbicides

• To produce a rice variety rich in Vitamin A

Which of the following is a circular DNA from bacteria that can hold a foreign gene?

• plasmid 

• chromosomes

• plastid 

• nucleus

Which of the following palindromic base sequence identify by EcoR1?

• 5' GATTTC 3’ 

• 5' GATTCC 3'

• 5' GAATCC 3’ 

• 5' GAATTC 3’

A biotechnology company plans to produce human insulin using bacteria. Which of the following steps would they first need to take to begin this process?

• Extract human insulin directly from human cells

• Insert the gene for human insulin into bacterial DNA

• Grow bacteria in large fermenters

• Harvest and purify the insulin from bacterial cultures