LABXPERT QUIZ 2

The first step in isolating and purifying nucleic acid.

• Elution

• Lysis

• Precipitation

• Denaturation

A proteolytic enzyme derived from Tritirachum album.

• protease

• polymerase

• proteinase K

• proteolenzyme

Which among the following is not included in organic isolation methods.

• phenol

• sodium acetate

• chloroform

• ethanol

TRUE or FALSE: Guanidine isothiocyanate (GITC) a strong denaturant of RNases and can be used instead of high salt buffers.

• TRUE

• FALSE

At which wavelength are nucleic acids read?

• 230

• 260

• 280

• 270

Is the extracted nucleic acid pure given the following 260/280 ratio of 1.9?

• YES

• NO

Is it the most sensitive method for determining nucleic acid purity and concentration yield.

• spectrophotometry

• electrophoresis

• nephelometry

• fluorometry

Which among the following is not a step in PCR?

• Adsorption

• Denaturation

• Annealing

• Elongation

The polymerase used in PCR is derived from what microorganism?

• Bacillus stearothermophilus

• Bacillus anthracis

• Thermus aquaticus

• Trichirium aquaticum

Method used to separate molecules based on their net charges and molecular weight.

• spectrophotometry

• nephelometry

• electrophoresis

• fluorometry