MBT QUIZ2

What is the minimum time required for denaturation in thermal-cycle PCR?

• One minute

• 30 seconds

• Less than one second

• 90 seconds

Nested PCR involves using two sets of primers

• True

• False

Which of the following ends of a PCR primer can be extended by polymerase?

• The 1’ end

• The 2’ end

• The 3’ end

• The 5’ end

By running the polymerase chain reaction overnight for twenty-one cycles, a single segment of DNA can be amplified to around a thousand copies.

• True

• False

The PCR can be used to clone genes from one organism by using priming sequences from another if some sequence data are available for the gene.

• True

• False

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

• The PCR reaction would not commence

• The reaction would yield a mixture of non-specific products

• The PCR reaction would end after one cycle

• The reaction would generate a single short PCR product

The melting temperature is the temperature at which theDNA disintegrates into it’s separate nucleotides?

• True

• False

The primers should flank the target sequence

• True

• False

Polymerase chain reaction(PCR) is a method for the selective amplification of DNA sequences.

• True

• False

Thermostable DNA polymerases such as Taq polymerase have enabled automation of the PCR process without the need for adding fresh polymerase after the denaturation stage of each cycle.

• True

• False