DG MIDTERM REVIEW QUIZ

How much agarose would you need in order to make a 2% gel with 175mL TAE?

• 2.75g

• 3.5g

• 3.25g

• 2.25g

Order the steps of PCR

• denaturation

• extension

• annealing

Which of these polymorphisms may cause significant difference in band size from the template strand? (choose all that apply)

• 230 SNPs at various locations which cause the translated protein to be non-functional

• A 400 bp transposable element flanked by TIRs

• A 750 bp deletion in the middle of the strand

• A 5 bp insertion in the middle of the strand

Find the amount of water and 5x tax needed for this PCR reaction

• 23.7 uL water, 7.3 uL Taq

• 24.6 uL water, 6.4 uL Taq

• 23.4 uL water, 7.6 uL Taq

• 24.8 uL water, 6.2 uL 5X Taq

How much DNA should be added to the master mix?

• 3 uL

• 0 uL

• 1.5 uL

• 4 uL

Say you have 5 DNA samples that you want to amplify via PCR. How many reactions should you prepare you master mix for?

• 5 reactions

• 6 reactions

• 7 reactions

• 8 reactions

How many copies of PCR product will you produce with 15 cycles?

• 25642

• 35048

• 100000

• 32768

You have performed bacterial transformation with a vector plasmid which contained a kanamycin resistance gene and a gene which produces blue fluorescent proteins when exposed to sucrose. Match the following results and the plates which may produce those results.

• Plate treated with LB+Kanamycin

• A lawn of bacteria (abundant bacteria growth)

• Plate treated with LB and no kanamycin

• Bacteria colonies that do not fluoresce blue

• Plate treated w/ LB + kanamycin+sucrose

• Bacteria colonies that fluoresce blue

Which enzyme is specifically needed for the production of cDNA?

• DNA polymerase

• reverse transcriptase

• mRNA polymerase

• Topoisomerase

Why are smaller DNA fragments able to move further away from the wells in gel electrophoresis?

• smaller DNA fragments are more physically able to move through the agarose gel's matrix thus they travel further

• the negative charge is stronger on smaller DNA fragments thus the electric repulsion causes more movement

• the ethidium bromide is able to pull smaller DNA fragments more easily due to less total molecular weight

• they aren't, larger DNA strands move further away from the wells as heavier molecular weight and charge enable larger DNA fragments to move further