RBC and WBC Count

Is is the number of red cells in mm³ of blood

(a)  

Blood is diluted with an i_ s_ to preserve the blood corpuscles

(a)  

Give 3 characteristics of a good diluting fluid of RBCs

• must be an isotonic solution

• must be hard to prepare

• must be expensive

• must have a high specific gravity

• must be easy to prepare

U_ s_ is a conventional method of preparing diluted blood samples for cell counting. A specific amount of 10 microliters of blood is collected using the capillary pipette.

(a)  

In the pipette method procedure, aspirate blood up to the _._ mark of the RBC pipette

(a)  

In the pipette method procedure of RBC, use syringe aspiration to draw the diluting fluid up to the (a)   mark with a constant rotation of the pipette to ensure proper mixing of the blood and diluting fluid. This makes 1:200 or 1/200 dilution.

In the pipette method procedure, gradually bring the pipette into a _ position and immediately mix the contents of the pipette. The pipette must be held securely using the _ and _ finger.

(a)  

In the pipette method procedure, i_ n_ counting chamber is used.

(a)  

In the pipette method procedure for RBC, discard the first _-_ drops of the diluted blood. The angle of the pipette while charging is _-_ degree.

(a)  

In the pipette method procedure, o_ can be observed by the presence of fluid on the moats of the counting chamber and u_ is evidenced by the failure to cover the entire ruled area of the counting chamber.

(a)