Sequencing 06 Nov 2023

What are the two methods in basic sequencing technique?

• Chemical Degradation method & Shotgun method

• Maxam-Gilbert method & Sanger method

• Chain Termination method & Sanger method

• Shotgun method & Sanger method

Piperidine is used to cut...

• Phosphodiester bond

• Hydrogen bond

• Glycosidic bond

• Ribose bond

Bioinformatics requires complete genome sequences to be useful.

• TRUE

• FALSE

What is the specific format used to copy our sequence into BLAST?

• NGS

• FASTA

• MEGA

• SEQ

What will dimethyl sulfate methylate in an acidic environment?

• Pyrimidine

• Cytosine

• Guanine

• Purine

When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and _____.

• Forward AND reverse primers

• Forward AND reverse probes

• Forward OR reverse primers

• Forward OR reverse probes

What is the sequence of the DNA fragment from the image?

Automated DNA sequencing reactions are done in a single tube because each dNTPs is labeled with a different fluorescent dye.

• TRUE

• FALSE

In Sanger method, we adopted the PCR technique and electrophoresis with agarose gel to amplify the DNA template and separate the fragment, respectively.

• TRUE

• FALSE

Which one from the molecules below is the ddNTP molecule?

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