MICROSCOPY

How many objectives are typically attached in a compound microscope?

If the image of the specimen is blurry, which part of the microscope will you adjust?

• body tube

• coarse adjustment knob

• fine adjustment knob

• mirror

What do you call the circular area you see under the microscope?

If the eyepiece has a magnification of 5x and I will use my scanner objective with a magnification of 4x, what is the total magnification of the specimen when viewed under the microscope?

Which is the correct arrangement of manipulating the microscope?

a. Adjust the body tube upwards using the coarse knob to insert the slide.

b. Lock the glass slide in place using the stage clips.

c. check the microscope if it is in the standard position.

d. Look into the eyepiece while adjusting the mirror to ensure there is enough light in the field of magnification.

e. While looking into the eyepiece, slowly adjust the body tube until a clear view of the specimen is seen.

• c, d, a, b, e

• c, a, d, b, e

• c, a, b, e, d

• c, d, e, a, b

When the field of magnification is dark, but the mirror is properly adjusted, which can possibly be the problem?

• The body tube is too high.

• Wrong objective is used.

• Diaphragm must be closed.

• The specimen is not positioned properly.

When beginning to use the microscope, which objective must be used first?

Which two parts must be held when carrying the microscope?

• arm and base

• arm ang stage

• base and body tube

• base and body tube

What do you call a slide/specimen that is prepared right before you observe under the microscope?

What is the correct arrangement of preparing a wet mount?

( Separate your answer with a comma, do not use space)

a. Put the specimen on top of the water on the glass slide.

b. Slowly lower the cover slip over the specimen.

c. Place 2-3 drops of water on a glass slide.