Introduction to Micropipettes Quiz

What type of pipette is this?

• Micropipette

• Transfer pipette

• Volumetric Pipettes

What type of pipette is this?

• Micropipette

• Transfer pipette

• Volumetric Pipettes

What type of pipette is this?

• Micropipette

• Transfer pipette

• Volumetric Pipettes

What materials do we need to collect? (Select all that apply)

• Beaker

• Micropipette

• Test tube

• Tips

• Tips box

Tags

NGSS.MS-PS1-2

Why is it important to set the correct measurement on the pipette?

• Helps to give an accurate measurement and to prevent the breakage of pipettes

• Helps to give an accurate measurement and to prevent breakage of pipettes

• Helps to give an accurate measurement and to break the pipettes

• Helps to give an inaccurate measurement of the liquid and brake the micropipettes.

When using a micropipette to pick up liquid:

What are we trying to avoid?

At what point do we press the plunger?

(select all that apply)

• Bubbles

• First stopping point

• Second stopping point

• Extra liquid

What step is this:

Collect Micropipette

Collect Pipette Tip box

Beaker, a test tube, or a centrifuge. (Depending on what you need for the lab)

• Step 1 Collect Materials

• Step 2: Setting Quantity

• Step 3
  Adding pipette tip

• Step 4
  Picking up liquid

• Step 5
  Releasing the liquid

  &

  Step 6
  Degrading the pipette tip

Which of the following is step 6: Degrading the pipette tip?

• 1. Hold Micropipette with forefinger under the ergonomic grip. Wrap fingers around, leave thumb off the plunger.

  2. Gently press plunger to the first stopping point.

  3. Elbow on the table. Pipette straight up and down into the beaker.

  4. Hold just above the bottom of the beaker.

  5. Slowly let off the plunger.

  - goal not get air bubble

• Remove thumb from plunger.

  Hold pipette over the tip collection bucket.

  Place the pipette as close as possible to the collection bucket. Then slowly press the tip ejector button.

  - To prevent bleach solution splash back.

• Take out of the beaker.

  Then with elbow on the table, hold over the center of a test tube, beaker, or centrifuge to place the liquid.

  Place pipette tip as close as possible to the bottom of the equipment without touching.

  Press to first stop. Hold then press to second stop. Remove pipette.

• Hold Micropipette with forefinger under the ergonomic grip. Wrap fingers around, leave thumb off of the plunger.

  Only Remove lid enough to have access to a tip. In order to keep a sterile environment.

  Hold directly over the center of the tip. Place pipette end into the tip opening. Then Press firmly into the tip. Until it clicks.

  Left out and close lid.

  - Do not touch the tip with hands. To maintain a sterile environment.

Which of the following is Step 3 Adding pipette tip?

• 1. Hold Micropipette with forefinger under the ergonomic grip. Wrap fingers around, leave thumb off the plunger.

  2. Gently press plunger to first stopping point.

  3. Elbow on the table. Pipette straight up and down into the beaker.

  4. Hold just above the bottom of the beaker.

  5. Slowly let off the plunger.

  - goal not get air bubble

• Remove thumb from plunger.

  Hold pipette over the tip collection bucket.

  Place the pipette as close as possible to the collection bucket. Then slowly press the tip ejector button.

  - To prevent bleach solution splash back.

• Take out of the beaker.

  Then with elbow on table, hold over the center of a test tube, beaker, or centrifuge to place the liquid.

  Place pipette tip as close as possible to the bottom of the equipment without touching.

  Press to first stop. Hold then press to second stop. Remove pipette.

• Hold Micropipette with forefinger under the ergonomic grip. Wrap fingers around, leave thumb off of the plunger.

  Only Remove lid enough to have access to a tip. In order to keep a sterile environment.

  Hold directly over the center of the tip. Place pipette end into the tip opening. Then Press firmly into the tip. Until it clicks.

  Left out and close lid.

  - Do not touch the tip with hands. To maintain a sterile environment.

Which of the following is Step 5
Releasing the liquid

• 1. Hold Micropipette with fore finger under the ergonomic grip. Wrap fingers around, leave thumb off the plunger.

  2. Gently press plunger to first stopping point.

  3. Elbow on the table. Pipette straight up and down into the beaker.

  4. Hold just above the bottom of the beaker.

  5. Slowly let off the plunger.

  - goal not get air bubble

• Remove thumb from plunger.

  Hold pipette over the tip collection bucket.

  Place the pipette as close as possible to the collection bucket. Then slowly press the tip ejector button.

  - To prevent bleach solution splash back.

• Take out of the beaker.

  Then with elbow on table, hold over the center of a test tube, beaker, or centrifuge to place the liquid.

  Place pipette tip as close as possible to the bottom of the equipment without touching.

  Press to first stop. Hold then press to second stop. Remove pipette.

• Hold Micropipette with fore finger under the ergonomic grip. Wrap fingers around, leave thumb off of the plunger.

  Only Remove lid enough to have access to a tip. In order to keep a sterile environment.

  Hold directly over the center of the tip. Place pipette end into the tip opening. Then Press firmly into the tip. Until it clicks.

  Left out and close lid.

  - Do not touch the tip with hands. To maintain a sterile environment.