Blood Culture

Which of these are correct for BACT/ALERT® VIRTUO®?

• Revolutionary Load and Go technology provides hands free bottle loading and unloading

• Receive Real-Time notification sample volume & 360° bottle scanning

• Faster time to detect blood stream infection by 20%

•   Bottle & instrument reports available 24/7 & Deeper insight with Maestria

• All of the above.

Answer explanation

Inadequate blood sample volume in Blood Culture procedure may hamper the microbial growth resulting in delayed accurate treatment.

BACT/ALERT^® VIRTUO^® facilitates real-time blood volume measurement, which allows to act immediately when needed; and monitors blood collection practice.

“Load & Go Technology”

Due to heavy work load there may be a delay in Blood Culture bottles loading in system which delays the growth of microorganism.

BACT/ALERT^® VIRTUO^® comes with revolutionary “Load & Go” technology which reduces hands on time, eliminates barrier of delayed bottle loading and removes negative bottles spontaneously for continuing free up space to accommodate additional bottle capacity

BACT/ALERT^® bottles are capable of detecting?

• Bacteria, Virus, Yeast

• Bacteria, Virus, Parasites

• Bacteria, Mycobacteria and Yeast

• Virus, Mycobacteria and Yeast

Answer explanation

BACT/ALERT bottles are USFDA approval improves which improves  patients safety.

During Sepsis management every hour is important, therefore microbial growth-culture media for small sample volume-sterile body fluids is necessary for best patient practices.

BACT/ALERT® FAN Plus® media is extensively studied & validated on clinical samples for Sterile Body Fluids(CSF, Synovial fluid, Peritoneal fluid, etc) and is US FDA approved to be used on Blood and SBFs, which enhances patient safety and peace of mind to Microbiologist and Clinicians.

As per the CUMITECH guideline, How much volume to be drawn for Blood Culture?

• 10 ml in Single Aerobic Bottle only

• 20 to 30 ml divided in 2 bottles of Aerobic and Anaerobic Bottles

• 15 ml in divided in 2 Aerobic bottles.

• 8-10 ml divided in 2 bottles of Aerobic only.

Answer explanation

Single Bottle V/S Paired Blood Culture practice-Blood Culture remains Gold Standard for detecting Bacteraemia. However, single bottle blood culture may hamper the clinical decision making due to non-specific isolation of pathogens. As per the CUMITECH guideline, the recommended volume of blood culture for investigating adults is 20-30 mL divided into 2 bottles, typically an anaerobic and an aerobic bottle which improves detection sensitivity.

Addition of BACT/ALERT 3D FN plus (Anaerobic bottle) in blood culture provides..

• Double the volume which increases the positivity rate.

• Improved detection of facultative anaerobes (e.g. streptococci, Enterobacterales)

• Improves the antibiotic neutralization.

• Increase coverage for Strict Anaerobic pathogens

• All of the above.

Answer explanation

•        Recovery of 704 clinically significant isolates from 2 aerobic bottles vs
1 aerobic bottle and 1 anaerobic bottle¹

•        Isolates

•        69.2% recovered from one or both bottles in each pair of bottles

•        12.2% recovered only from the FAN aerobic-FAN aerobic pair

•        18.6% recovered only from the FAN aerobic-FAN anaerobic pair

•        Significantly more total organisms (P = 0.002), Gram-positive cocci (P = 0.003),
S. aureus (P = 0.005), Enterobacteriaceae other than E. coli (P = 0.002) and anaerobes (P = 0.01) from the FAN-aerobic-FAN anaerobic pair than from the FAN aerobic-FAN aerobic pair

•        Significantly more S. aureus (P = 0.005) and anaerobes (P < 0.001) were recovered from the FAN anaerobic bottle

•        These results support the routine use of the FAN anaerobic bottle for use in the culture of blood

Other advantages of Anaerobic culture

•        Double volume of blood that is cultured

•        Recovery of anaerobes

•        Improved detection of facultative anaerobes (e.g. streptococci, Enterobacterales)

•        Faster detection in anaerobic conditions

•        Detection only in anaerobic bottle (16% of streptococci and 17% of Enterobacterales

•        Although an aerobe, a recent study showed Recovery of C.glabrata is faster in anaerobic blood culture

Which type of BACT/ALERT^® 3D bottles contain Adsorbent Polymeric Beads (APB) for faster antibiotic neutralization?

• BACT/ALERT FA plus

• BACT/ALERT FN plus

•    BACT/ALERT PF plus

• All of the above

Answer explanation

Antibiotic Neutralization during Blood Culture process.

Faster Antibiotic Neutralization is an important phase of microbial growth process in Blood Culture, which is very significant to reduce false negatives and improve patient safety.

BACT/ALERT® FAN® Plus media is enriched with Adsorbent Polymeric Beads which is most effective in Antibiotic Neutralization and improves patient safety.