BIOL 475 Exam 4 Review

What is not a proofreading mechanism for aminoacyl-tRNA synthetase?

• size exclusion

• editing post-transfer

• editing pre-transfer

• wobble pairing

Once the amino acid is activated by attachment of AMP, then the aminoacyl-tRNA transferase can...

• attach activated aminoacyl-adenylate to the 2' or 3' OH ribose of adenine

• attach activated aminoacyl-adenylate to the 3' OH ribose adenine

• attach activated aminoacyl-adenylate to the 2' OH ribose adenine

• attach activated aminoacyl-adenylate to the 5' CCA tail

The Svedberg unit for the small ribosomal subunit in eukaryotes is...

• 30S

• 40S

• 50S

• 60S

Bacterial RF1 and 2 recognize UAA (eRF1 recognizes all stop codons). What other stop codon does RF1 recognize?

• AUG

• UGA

• UAG

• AUA

What are the respective orthologs of IF1 and IF3 for eukaryotes?

• eIF1 & eIF1a

• eIF1a & eIF1

• eIF1 & eIF3

• eIF3 & eIF1

What protein translocated the tRNAs from the A site to the P site?

• EFTu

• EF-G/eEF2

• eIF2

• IF2

What is eIF2's and eIF5B's function?

• to bind to the A site

• to bind to the E site

• to hydrolyze GTP for recycling large and small subunits

• to hydrolyze GTP for joining the small and large subunits

Recognition of the AUG is sensitive to what sequence context in eukaryotes?

• Shine Dalgarno Sequence

• Anti-Shine Dalgarno Sequence

• Kozak Sequence

• Anti-Kozak Sequence

What's the function of eIF4e and PABP in translation?

• bind to cap and tail to form loop

• bind to cap and tail to avoid overtranslation

• unwind secondary structures

• catalyze large subunit joining

In bacteria, what promotes the dissociation of RF1/2 after peptide release?

• RF2-GTP

• RF1

• RF3-GTP

• RF3