Revision Fall 2023 Chromatographic techniques and cell culture22

___________ is the purpose of passaging cells in a cell culture lab.

• To increase the cell density

• To maintain cells in a logarithmic growth phase

• To decrease cell viability

• To induce cell differentiation

Trypsin is used in cell culture for subculturing to:

• color cells for microscopic analysis

• induce apoptosis

• detach cells from the culture surface

• enhance cell adhesion

"Confluence" refers in the context of cell culture to:

• The number of dead cells in the culture

• The growth rate of cells

• The percentage of the culture surface covered by attached cells

• The process of cell fusion

The role of DMSO (Dimethyl sulfoxide) in cell culture is to:

• Enhance cell adhesion

• Prevent contamination

• Cryopreserve cells

• Stimulate cell growth

__________ is the primary driving force in paper chromatography.

• Centrifugal force

• Capillary action

• Magnetic force

• Gravitational force

The role of the mobile phase in HPLC is to:

• Dissolve the sample

• Carry the sample through the column

• Interact with the stationary phase

• Stabilize the column temperature

___________ is the basis of separation in affinity chromatography.

• Size of molecules

• Charge of molecules

• Specific binding interactions

• Hydrophobic interactions

_______ is responsible for generating the high pressure needed for efficient separation in HPLC.

• Pump

• Injector

• Detector

• Column