Restriction enzymes + Cloning

Bacterial DNA is protected from their own restriction enzymes due to presence of

• Methylase enzyme

• Endonuclease enzyme

• Exonuclease enzyme

• None of the above

Different restriction enzymes that recognize and cleave in the same location of DNA are known as

• Neoschizomers

• Isoschizomers

• Non-palindromic

• Palindromic

Restriction enzymes that cut phosphodiester bonds and hydrogen bonds between nucleotides result in formation of

• Blunt ends

• Sticky ends

• Both are correct

• None of the above is correct

The star activity of restriction enzyme results from

• Keeping enzymes in 50% glycerol before digestion

• using 5% glycerol concentration during digestion

• Using low enzyme concentration

• Prolonged digestion time

Restriction enzymes could be used in

• Gene cloning

• Plasmid propagation

• Distinguishing gene alleles

• All of the above

Multiple cloning site:

• Contains start codon of the plasmid

• Part of the cloned gene

• Part of the plasmid used for cloning

• Contains promoter of the plasmid

Different restriction enzymes that recognize same site but cleave in different locations in that site, are known as

• Neoschizomers

• Isoschizomers

• Palindromic

• Non-palindromic

Restriction sites added to primers used for cloning are:

• Present in MCS of the plasmid

• Present in the middle of cloned gene

• Both are correct

• None is correct

Cloning:

• Propagating a certain gene inserted into a plasmid

• Used for protein expression

• Both

• None of the above

Restriction enzymes that cut phosphodiester bonds only between nucleotides result in formation of

• Sticky ends

• Blunt ends

• Both

• None of the above