Theta replication is unidirectional. T or F?

• T
• F

Intron Branch point: a conserved “A” residue located ___ nucleotides upstream of 3’ splice site (If your answer is 15 to 19 for example, type your answer as "15-19", not including the quotation marks)

Poly A consensus sequence (AAUAAA) is the polyadenylation signal located ___ nucleotides upstream of the cleavage site (If your answer is 15 to 19 for example, type your answer as "15-19", not including the quotation marks).

Amino acid attachment to tRNA's occur at the...

• 3' end
• 5' end

In eukaryotes, termination of transcription by RNA Polymerase _ is similar to rho independent termination in prokaryotes (ends after transcribing a terminator sequence that produces

a string of U’s that is downstream of a hairpin)

• RNA Polymerase I

• RNA Polymerase II

• RNA Polymerase III

In eukaryotes, termination of transcription by RNA Polymerase _ is similar to rho dependent termination in prokaryotes (requires a termination factor similar to the rho factor)

• RNA Polymerase I

• RNA Polymerase II

• RNA Polymerase III

In eukaryotic transcription with RNA polymerase _, transcription continues past termination sequence, with RNA being cleaved at a consensus sequence in the RNA.

• IV
• V
• II
• I

In eukaryotic transcription with RNA polymerase II, the Rat1 protein has Rat 1 ’_ to _’ exonuclease activity and attaches to the _’ end to chew up the unnecessary RNA.

• 3' to 5', 5'

• 5' to 3', 3'

• 3' to 5', 3'

• 
  

  5' to 3', 5'

Which of the following is specific to eukaryotic mRNA?

• PolyA tail

• 5' cap

• introns

In eukaryotes, splicing of introns takes place in the...

• cell membrane
• mitochondria
• nucleus
• cytoplasm