1.1.6. Electrophoresis Quiz

If you are making a 30 mL gel, how much concentrated buffer should you add? I want the answer in MICROLITERS, since you'll be using the micropipetter. Type the answer with NO unit symbol after it.

(a)  

If you are making a 0.8% 30 mL gel, how much agarose powder should you add? The answer will be in grams. Type the answer with NO unit symbol after it.

(a)  

Approximately how long will you need to microwave the mixture?

• 10 seconds
• 1 minute
• 2 minutes
• 5 minutes

How many wells should the combs we use have?

• 6
• 8
• 10
• 12

If we're making a 30 mL gel, how many microliters of DILUTED SYBR Safe should be added? Type the answer without a unit.

(a)  

When should you pour the mixture into the gel tray

• As soon as it comes out of the microwave
• As soon as it reaches 37 degrees C
• As soon as it's cool enough to hold in the palm of your hand
• Once it reaches room temperature

When is the SYBR Safe added?

• before microwaving mixture
• after microwaving mixture & before pouring it
• after pouring mixture into gel tray
• when loading DNA into gel

If using your gel the next day, how should it be stored?

• on the counter at room temperature
• in the incubator at body temperature
• in the refrigerator
• in the electrophoresis apparatus at room temperature

How many mL of running buffer will fill our M12 chamber? Type the answer without a unit.

(a)  

To make our running buffer, how many mL of concentrated buffer should we add? Type the answer without a unit.

(a)