Using the Microscope

An animal cell being observed is seen at the topmost part of the field of view under the Low Power Objective (LPO). If you want to position the specimen at the center, which direction should you move the slide?

• Downward or towards me or the user

• Forward or away from me or the user

  
  

Julia was observing an onion cell under the High Power

 Objective (HPO) and she noticed that it moved upward and then it moved to the right. What does this tells Julia about the actual movement of the specimen?

• The specimen actually moved downward and to the left.

• The specimen moved downward and to the right.

  
  

 When looking at letter “e” under the microscope, what is the position of the letter as seen under the microscope?

• The position stays the same.

•  The letter “e” appears larger and inverted.

The following statements describe how to focus a specimen under the compound microscope, EXCEPT:

•  Aim the mirror at your light source.

• Use the fine adjustment knob to bring the specimen into focus.

• Adjust the stage in order to find the center focus of the specimen.

• Slowly move the coarse adjustment knob towards you until the specimen becomes invisible.

 A plant cell is viewed using a 10x eyepiece magnification and 43x HPO. How many times will it be magnified?

•  43 times 

• 430 times

 Tilting the microscope allows one to do observation while sitting. Which of the following is not applicable when a microscope is tilted?

• Viewing a protists in water

• Viewing an onion skin

Which of the following describes a microscope’s magnification?

• A microscope’s ability to enlarge an image of the object’s length in one direction but not changing the actual size.

• A microscope’s ability to generate an image without changing sizes.

Which type of objective are you going to use in focusing a specimen if the inscription is 100x or 97x magnification?

•  Oil Immersion Objective

• Scanner

 How will you be able to know that the revolving nosepiece of the HPO is properly aligned with the eyepiece?

• The objective produces a “click” sound when the revolving nosepiece is turned.

• The revolving nosepiece of the objective turns easily.

If you wanted to view bacteria, protists and fungi, which objective are you going to use?

• Scanner

• Oil Immersion Objective