CRISPR Cas9 PreAssessment

Explain the function of CRISPR Cas9 in gene editing.

• It is a tool for repairing car engines

• It is a tool used for precise gene editing by cutting DNA at specific locations.

• It is a type of plant fertilizer

• It is used to treat skin diseases

What are the potential applications of CRISPR Cas9 technology?

• Medical research, agriculture, and gene therapy

• Cooking recipes, fashion design, and car manufacturing

How does CRISPR Cas9 perform gene editing at the molecular level?

• By using a guide RNA to target a specific DNA sequence and then cutting the DNA at that location

• By using sound waves to disrupt the DNA sequence

• By using a laser to remove the targeted DNA sequence

• By injecting a chemical that alters the DNA structure

Describe the components of CRISPR Cas9 structure.

• Cas9 protein, guide RNA, and target DNA sequence

• Cas9 protein, guide RNA, and non-target DNA sequence

• CRISPR protein, guide DNA, and target RNA sequence

• Cas10 protein, messenger RNA, and non-target DNA sequence

Explain the mechanism of CRISPR Cas9 function in gene editing.

• CRISPR Cas9 uses guide RNA to target specific DNA sequences and the Cas9 enzyme to cut the DNA at the targeted location.

• CRISPR Cas9 uses guide RNA to target random DNA sequences and the Cas9 enzyme to repair the DNA at the targeted location.

• CRISPR Cas9 uses guide DNA to target specific DNA sequences and the Cas9 enzyme to cut the DNA at the targeted location.

• CRISPR Cas9 uses guide RNA to target specific RNA sequences and the Cas9 enzyme to cut the RNA at the targeted location.

The gray area is ______ which is composed of ________

The blue strant is ______ which is composed of ________

What is the significance of the Cas9 enzyme in the CRISPR Cas9 system?

• It acts as a guide RNA

• It is responsible for cutting the DNA at the targeted location

• It helps in the replication of DNA

• It has no specific role in the CRISPR Cas9 system

What is the role of guide RNA in the CRISPR Cas9 system?

• It helps in the replication of DNA

• It guides the Cas9 enzyme to the specific DNA sequence to be edited

• It acts as a messenger carrying information from DNA to the ribosome

• It has no specific role in the CRISPR Cas9 system

What are the limitations of using CRISPR Cas9 for gene editing?

• It can only edit genes in plants

• It can cause off-target effects, altering non-targeted genes

• It can only edit genes in animals

• There are no limitations to using CRISPR Cas9 for gene editing