MI 4.1.2 Module 4 Pre-Lab

Why are you not supposed to boil the GFP native solution?

• we want to denature it

• it is already denatured

• it will help us compare native and denatured proteins after electrophoresis

Tags

NGSS.HS-PS3-4

What two chemicals does the denaturing solution contain?

• SDS

• 2-mercaptoethanol

• DTT

• CSS

• NPS

You will boil your "GFP Denatured" sample at ​ (a)   degrees C for ​ (b)   minutes and then allow them to cool. You must ALWAYS use ​ (c)   tubes when boiling samples.​ ​

• 95-100
• 5
• screw-top
• 10
• 2
• flip-top
• uncapped
• 37
• 212

Tags

NGSS.HS-PS3-4

Denatured GFP will glow green under UV light.

• true

• false

What will you CAREFULLY remove from the gel after you open it? (Choose all that apply.)

• tape at the bottom

• the comb

• buffer

• air

The short plate on the gel cassette should face ________ the gel cassette clip.

• toward

• away from

Fill the chamber with buffer until it covers the

• shorter plate

• taller plate

• top edge of the chamber

• bottom of the chamber

How much sample, in uL, will you place into each well of the gel?

Mathematical Equivalence

ON

Run electricity through the gel until the (a)   dye runs to the bottom of the cell.

Tags

NGSS.HS-PS3-5

If you run the electricity at 125 volts, what is the optimal time will it take to complete the electrophoresis?

Mathematical Equivalence

ON