MI 4.1.2 Module 4 Pre-Lab

Why are you not supposed to boil the GFP native solution?

• we want to denature it

• it is already denatured

• it will help us compare native and denatured proteins after electrophoresis

Tags

NGSS.HS-PS3-4

What two chemicals does the denaturing solution contain?

• SDS

• 2-mercaptoethanol

• DTT

• CSS

• NPS

You will boil your "GFP Denatured" sample at ​ (a)   degrees C for ​ (b)   minutes and then allow them to cool. You must ALWAYS use ​ (c)   tubes when boiling samples.​ ​

• 95-100
• 5
• screw-top
• 10
• 2
• flip-top
• uncapped
• 37
• 212

Tags

NGSS.HS-PS3-4

Denatured GFP will glow green under UV light.

• true

• false

What will you CAREFULLY remove from the gel after you open it? (Choose all that apply.)

• tape at the bottom

• the comb

• buffer

• air

The short plate on the gel cassette should face ________ the gel cassette clip.

• toward

• away from

Fill the chamber with buffer until it covers the

• shorter plate

• taller plate

• top edge of the chamber

• bottom of the chamber

How much sample, in uL, will you place into each well of the gel?

Mathematical Equivalence

ON

Run electricity through the gel until the ________ dye runs to the bottom of the cell.

Tags

NGSS.HS-PS3-5

If you run the electricity at 125 volts, what is the optimal time will it take to complete the electrophoresis?

Mathematical Equivalence

ON