PCR Quiz

What does PCR stand for?

• Polymerase Chain Reaction

• Preliminary Chromosome Reconstruction

• Pathogen Containment Regulation

• Protein Coding Region

Explain the process of PCR.

• PCR is a technique used to amplify a specific segment of DNA through a series of temperature cycles.

• PCR is a technique used to measure the concentration of proteins in a sample.

• PCR is a process used to separate different components of a DNA sample.

• PCR is a method for creating new species of plants through genetic modification.

What are the three main steps of PCR?

• Denaturation, Annealing, Extension

• Hybridization, Ligation, Transformation

• Separation, Purification, Amplification

• Mixing, Heating, Cooling

What is the purpose of denaturation in PCR?

• To introduce mutations in the DNA

• To separate the DNA strands for primer binding and DNA polymerase activity.

• To prevent DNA replication

• To increase the stability of DNA strands

What is the role of primers in PCR?

• Primers are used to identify the end of the DNA strand

• Primers have no role in PCR

• Primers help initiate DNA synthesis in the target region

• Primers prevent DNA synthesis in the target region

Why is PCR important in genetic testing?

• Identifying protein structures

• Measuring blood pressure

• Counting red blood cells

• Amplification of specific DNA sequences

What is the significance of PCR in forensic science?

• PCR is used to identify suspects in forensic science

• PCR is used to analyze fingerprints in forensic science

• PCR is used to determine the cause of death in forensic science

• PCR is used to amplify small amounts of DNA for analysis in forensic science.

How does PCR contribute to the study of inherited diseases?

• PCR amplifies specific DNA sequences for easier detection of genetic mutations.

• PCR reduces the accuracy of genetic testing

• PCR creates new genetic mutations

• PCR has no impact on the study of inherited diseases

What are the limitations of PCR?

• No need for specific primers

• Ability to amplify long DNA fragments easily

• Limitations of PCR include potential for contamination, need for specific primers, and inability to amplify long DNA fragments.

• Unlimited amplification potential

Explain the concept of quantitative PCR (qPCR).

• A technique used to visualize DNA under a microscope.

• A technique used to identify the shape of a DNA molecule.

• A technique used to measure the amount of protein in a sample.

• A technique used to measure the amount of a specific DNA sequence in a sample.