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Protein Purification and Quantification

Authored by Jakiah Choudhury

Biology

9th - 12th Grade

45 Questions

NGSS covered

Used 1+ times

Protein Purification and Quantification
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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the technique used to separate molecules on the basis of size or density by high speed spinning?

Homogenization

Centrifugation

Filtration

Chromatography

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the principle behind hydrophobic interaction chromatography (HIC)?

Separating molecules based on affinity

Separating molecules based on size/shape

Separating molecules based on charge

Separating molecules based on hydrophobicity

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which buffer is used to wash away proteins with few hydrophobic regions in HIC?

Equilibration buffer

Binding buffer

Elution buffer

Wash buffer

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of freeze-thaw in the lysis phase of protein purification?

To remove cellular debris

To break the cells open

To separate the protein from other molecules

To concentrate the cells

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why does human tears and dogs’ saliva contain high concentrations of lysozyme?

To break the cells open

To concentrate the cells

To remove cellular debris

To protect against bacterial infections

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Where would the enzymes, hormones, and vitamins be found after centrifugation of blood?

In the supernatant

In the column side

In the gel beads

In the pellet

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of the equilibration buffer in HIC?

To prime the beads in the column for hydrophobic interactions (changes its pH)

To wash off the protein

To expose hydrophobic regions of the protein

To wash away proteins with few hydrophobic regions

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