Transcription in Prokaryotes (8)

What is the central dogma of transcription?

• DNA to RNA to protein

• RNA to DNA to protein

• RNA to protein to DNA

• Protein to DNA to RNA

Which enzyme is responsible for the synthesis of all three types of RNAs in E-Coli?

• DNA polymerase

• RNA ligase

• DNA ligase

• RNA polymerase

What is the function of the σ subunit in RNA polymerase?

• Recognizes the promoter for synthesis initiation

• Binds & opens DNA template

• Determines the DNA to be transcribed

• Catalyzes polymerization

What is the consensus sequence centered about -10 nucleotides in the prokaryotic promoter?

• GCGC

• TATAAT

• ATAT

• TTGACA

Which termination method involves the binding of the ρ termination factor to the mRNA?

• σ-independent termination

• σ-dependent termination

• ρ-independent termination

• ρ-dependent termination

What is the first nucleotide on the RNA transcript during initiation of transcription?

• UTP

• ATP

• CTP

• GTP

What is the region at which a stable complex of DNA and RNA-polymerase is formed?

• Termination

• Upstream

• Promoter

• Transcribed region

Which consensus sequence is recognized by prokaryotic RNA polymerase-σ complex and is centered about -35 nucleotides?

• ATAT

• TTGACA

• TATAAT

• GCGC

What is the enzyme that moves along the DNA and extends the growing RNA chain during elongation?

• DNA ligase

• RNA polymerase

• RNA ligase

• DNA polymerase

What is the sequence in the nascent RNA that is self-complementary and is followed by a series of U bases in ρ-independent termination?

• Stem-loop structure

• Hairpin

• Inverted repeat

• Palindrome