Edexcel IAL CP4: Enzyme-Catalysed Reactions Quiz

What is the purpose of using a colorimeter in this experiment?

• To measure the absorbance of the solution

• To monitor the temperature of the water baths

• To mix the enzyme and substrate solutions

• To prepare the reference cuvette

What is the effect of temperature on the initial rate of enzyme-catalysed reactions?

• Temperature stops the reaction

• Lower temperature increases the initial rate

• Higher temperature increases the initial rate

• Temperature has no effect on the initial rate

What is the purpose of using pH buffers in this experiment?

• To measure the absorbance of the solution

• To mix the enzyme and substrate solutions

• To test the effect of pH on the initial rate

• To monitor the temperature of the water baths

What is the effect of enzyme concentration on the initial rate of enzyme-catalysed reactions?

• Enzyme concentration has no effect on the initial rate

• Enzyme concentration stops the reaction

• Higher enzyme concentration increases the initial rate

• Lower enzyme concentration increases the initial rate

What is the purpose of preparing different substrate concentration solutions?

• To mix the enzyme and substrate solutions

• To measure the absorbance of the solution

• To test the effect of substrate concentration on the initial rate

• To monitor the temperature of the water baths

What is the risk associated with hot water baths in this experiment?

• Cuts from sharp objects

• Scalding

• Spillage causing slippery surfaces

• Allergic reaction or respiratory problems

What precaution should be taken when handling trypsin?

• Take care in removing and replacing the water bath lid

• Wear gloves to avoid skin contact

• Keep away from edge of desk

• Wipe up any liquid spillages as soon as they occur

What is the purpose of setting the reference absorbance of the colorimeter to zero?

• To measure the temperature of the water baths

• To account for any absorbance from the cuvette itself

• To prepare the reference cuvette

• To mix the enzyme and substrate solutions

What is the effect of substrate concentration on the initial rate of enzyme-catalysed reactions?

• Substrate concentration stops the reaction

• Substrate concentration has no effect on the initial rate

• Lower substrate concentration increases the initial rate

• Higher substrate concentration increases the initial rate

What is the risk associated with liquids in this experiment?

• Scalding

• Spillage causing slippery surfaces

• Cuts from sharp objects

• Allergic reaction or respiratory problems