CRISPR-Cas9

What does CRISPR stand for?

• Clustered regularly interactive short palindromic repeats

• Clustered regularly interspaced short palindromic repeats

• Deoxyribonucleic acid

• Regularly interspaced short palindromic repeats

Tags

NGSS.HS-LS3-2

How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes

CRISPR-Cas9 was discovered in which of the following organisms?

• protists

• fungi

• bacteria

• viruses

CRISPR can change DNA in humans and animals.  What did scientists look at to help them create CRISPR?

(cosa hanno dovuto osservare gli scienziati per aiutarli nella creazione del CRISPR?)

• Sheep cloning

• Palindromic repeats

• Common Cold

• Bacteria fighting off viruses

The enzyme portion of CRISPR??- this process is called ________.

or What enzyme is used in CRISPR gene editing?

• CRISPR

• Replicase

• Lysozyme

• Cas9

Tags

NGSS.HS-LS1-1

What is one way CRISPR has already been used to change genes?

• Clone sheep

• Give people superpowers

• Cure animal diseases

• Military purposes

How does CRISPR differ from previous techniques of gene editing?

• slower, more expensive, but more accurate

• faster, cheaper, and more accurate

• faster, more expensive, more accurate

• slower, cheaper, less accurate

What is the definition of genetic engineering?

• Studying the DNA of plants and animals

• Making changes in the DNA code of living organisms

• The discovery of DNA Using DNA to reproduce organisms

• Using DNA to reproduce organisms

What does the cas9 protein do?

• cut viruses

• Cut RNA

• cut DNA

• ingest bacteria

What does the guide RNA do?

• destroy invaders like bacteriophages immediately

• cut invading pieces of RNA

• recognize invaders

• recognize sequences of DNA to be edited.