2/12:CRISPR-Cas9 System Quiz

What is the significance of CRISPR-Cas9 system in biotechnology?

• It is used for creating artificial intelligence

• It is a method for producing renewable energy

• It is used for cleaning water sources

• It allows for an unprecedented level of control in manipulating the genetic information of a living organism

When was the presence of CRISPR first reported in the Escherichia coli genome?

• 1987

• 1995

• 2000

• 2010

What are the short, repeated sequences of DNA nucleotides found within the genome of prokaryotes called?

• DNA-RNA

• Prokaryotic repeats

• Genetic elements

• CRISPR

What is the function of CRISPR in prokaryotes?

• It is responsible for protein synthesis

• It helps in cell division

• It is involved in energy production

• It is a key component of their adaptive immunity

Who were the first to propose that the bacterial CRISPR-Cas9 system could be used as a programmable toolkit for genome editing in humans and other animal species?

• Jennifer Doudna and Emmanuelle Charpentier

• Atsuo Nakata and his team

• Osaka University researchers

• Streptococcus bacterium scientists

What is the name of the single molecule formed by fusing crRNA and tracrRNA together?

• Single guide RNA (sgRNA)

• Double guide RNA (dgRNA)

• Quadruple guide RNA (qgRNA)

• Triple guide RNA (tgRNA)

What is the role of Cas9 protein in the CRISPR-Cas9 system?

• It helps in cell division

• It is responsible for energy production

• It is a nuclease enzyme capable of cleaving DNA at specific nucleotide linkages

• It is involved in protein synthesis

Which pathway repairs double-strand breaks in DNA by directly ligating without the need for a homologous template?

• Mismatch repair (MMR)

• Single-strand repair (SSR)

• Homology-directed repair (HDR)

• Non-homologous end joining (NHEJ)

What is the potential application of CRISPR in cancer immunotherapy?

• Cleaning water sources

• Producing renewable energy

• Creating artificial intelligence

• Genetically modifying immune T cells to recognize and kill cancer cells

What is the cause of acquired immunodeficiency syndrome (AIDS)?

• Herpes simplex virus

• Hepatitis B virus

• Influenza virus

• Human immunodeficiency virus (HIV)