SARS-CoV-2 Quiz

What does SARS-CoV-2 use to enter target cells?

• Polyclonal antibodies

• ACE2 receptor

• Spike-mediated entry

• Cryo-EM structures

Which of the following statements is true regarding the affinity of SARS-CoV-2 and SARS-CoV to ACE2?

• SARS-CoV-2 has a higher affinity to ACE2 than SARS-CoV.

• SARS-CoV has a higher affinity to ACE2 than SARS-CoV-2.

• SARS-CoV-2 and SARS-CoV bind with similar affinities to ACE2.

• Neither SARS-CoV-2 nor SARS-CoV can bind to ACE2.

What is the role of SARS-CoV polyclonal antibodies in relation to SARS-CoV-2?

• They facilitate the entry of SARS-CoV-2 into cells.

• They inhibit the spike-mediated entry of SARS-CoV-2 into cells.

• They have no effect on the entry of SARS-CoV-2 into cells.

• They increase the affinity of SARS-CoV-2 to ACE2.

Which cell line was used to demonstrate that ACE2 is a functional receptor for SARS-CoV-2 S pseudotyped viruses?

• VeroE6 cells

• BHK cells

• HEK293 cells

• A549 cells

What is the main target of neutralizing antibodies (Abs) against SARS-CoV-2?

• The membrane-anchored S2 subunit

• The receptor-binding domain(s) of the S1 subunit

• The ACE2 enzyme

• The N-linked glycans

Which of the following is a consequence of the cleavage at the S1/S2 boundary of SARS-CoV-2 S?

• It prevents the virus from attaching to the ACE2 receptor.

• It stabilizes the prefusion state of the virus.

• It facilitates the entry of the virus into susceptible cells.

• It decreases the infectivity of the virus.

According to the text, what is the role of the S2 subunit of SARS-CoV-2?

• It binds directly to the ACE2 receptor.

• It contains the receptor-binding domain(s).

• It contains the fusion machinery.

• It is the main target for vaccine design.

According to the kinetic analysis of hACE2 binding, which variant of the virus has a higher affinity for hACE2 as indicated by a lower Kd value?

• SARS-CoV-2 S

• SARS-CoV S

• Both have the same affinity

• The document does not provide this information

What is the main feature conserved among the 144 SARS-CoV-2 isolates sequenced to date but not in the closely related SARS-CoV?

• The furin cleavage site

• The receptor-binding domain

• The hACE2 binding affinity

• The spike protein structure

Which of the following amino acid substitutions is associated with the SARS-CoV-2 S variant according to the document?

• N439K

• Y442S

• L455F

• Q498Y