Production of insulin

Plasmids are considered _____________ as they are used as agents to transfer genetic material into other cells

what are the two insulin chains called?

• alpha and beta chains

• A and B chains

• heavy and ligth chains

• long and short chains

Including Amp^r in the plasmid is used to help determine whether the gene of interest has been correctly inserted.

• True

• False

What is a plasmid?

• A linear section of DNA that contains the gene of interest

• A small circular piece of DNA that is self-replicating

• A bacterium that has contains an inserted gene

• A specific sequence of bases that is cut by a restriction enzyme

A(n) _____________ such as lacZ allows for the confirmation that a plasmid is recombinant.

the gene of interest and the plasmid vector are always isolated from the same organism.

• true

• false

what will happen to untransfomed bacteria when they're put on X-gal and ampicillin?

• they turn blue!

• they grow exponentially

• They die

• They can make insulin

Electropolation involves using brief exposure of bacteria to an electrical field, to create holes in the plasma membrane and hence increase plasmid uptake

• ture

• false

provide two reasons as to why it is preferred that human insulin is manufactured rather than extracting the insulin from another oganism

Explain why the same recognition sites must be present on the ends of the gene of interest and at a known site in the selected plasmid.