QUALITY: HPLC

What does HPLC stand for?

• High-Performance Pumping Liquid Chromatography

• High-Performance Liquid Chromatography

• High Pressure Liquid Chromatography

• High-Pressure Pumping Liquid Chromatography

Answer explanation

HPLC stands for High-Performance Liquid Chromatography, so the correct choice is 'High-Performance Liquid Chromatography'.

What is the function of the high pressure pump in HPLC?

• To create a vacuum in the column

• To generate a low pressure for the column

• To regulate the flow rate of the mobile phase

• To generate a high pressure up to 40 mega Pascal's

Answer explanation

The function of the high pressure pump in HPLC is to generate a high pressure up to 40 mega Pascal's.

What material is the body column of HPLC made up of?

• Plastic

• Copper

• Aluminum

• Stainless Steel

Answer explanation

The column of HPLC is made up of Stainless Steel, which is the correct choice. It provides durability and resistance to corrosion, making it suitable for HPLC applications.

What is the purpose of the stationary phase in HPLC?

• To detect the sample components

• To generate high pressure

• To regulate the flow rate of the mobile phase

• To interact with the sample molecules

Answer explanation

The purpose of the stationary phase in HPLC is to interact with the sample molecules, allowing for separation based on different affinities.

What is the function of the detector in HPLC?

• To interact with the sample molecules

• To detect the sample components

• To regulate the flow rate of the mobile phase

• To generate high pressure

Answer explanation

The function of the detector in HPLC is to detect the sample components, making the second answer choice the correct one.

How are the sample molecules detected in HPLC?

• By the detector and a peak is obtained on the computer

• By the high pressure pump

• By the stationary phase

• By the solvent reservoir

Answer explanation

The sample molecules are detected in HPLC by the detector, and a peak is obtained on the computer, making the first choice the correct answer.

What is required for the detection of sample components in HPLC?

• Stainless steel column

• Standards

• High pressure pump

• Injector

Answer explanation

Standards are required for the detection of sample components in HPLC as they are used to identify and quantify the components in the sample.

What is needed to find the concentration of a sample using HPLC?

• Standard curve

• Column

• Injector

• Detector

Answer explanation

To find the concentration of a sample using HPLC, a standard curve is needed to determine the concentration of the sample based on its peak area.

What is the stationary phase in normal phase HPLC?

• Porous

• Ionic

• Nonpolar

• Polar

Answer explanation

The stationary phase in normal phase HPLC is polar, allowing for separation based on polarity differences in the sample components.

What is the basis of separation in size exclusion HPLC?

• Polarity

• Molecular size

• Pressure

• Molecular charge

Answer explanation

Size exclusion HPLC separates molecules based on their molecular size, allowing smaller molecules to pass through the column pores while larger molecules are excluded. Therefore, the basis of separation in size exclusion HPLC is molecular size.