QUALITY-TLC (VIDEO)

What is the thickness of the adsorbent material in thin-layer chromatography?

• 0.1 to 0.3 kilometers

• 0.1 to 0.3 meters

• 0.1 to 0.3 millimeters

• 0.1 to 0.3 centimeters

Answer explanation

The thickness of the adsorbent material in thin-layer chromatography is 0.1 to 0.3 millimeters.

Where is the sample applied on the plate in thin-layer chromatography?

• Near the bottom of the plate

• Near the middle of the plate

• Near the top of the plate

• In the center of the plate

Answer explanation

In thin-layer chromatography, the sample is applied near the bottom of the plate to allow for proper separation and movement of the components.

What causes the separation of sample molecules in thin-layer chromatography?

• Difference in interaction with the mobile phase and the stationary phase

• Difference in color

• Difference in pressure

• Difference in temperature

Answer explanation

The separation of sample molecules in thin-layer chromatography is caused by the difference in interaction with the mobile phase and the stationary phase.

What method is commonly used to detect analytes in thin-layer chromatography?

• Infrared light

• X-ray

• Visible light

• Ultraviolet light

Answer explanation

Ultraviolet light is commonly used to detect analytes in thin-layer chromatography.

How are colored samples visualized in thin-layer chromatography?

• By using a developing chamber

• By using ultraviolet light

• By using a microscope

• By using a glass slide

Answer explanation

Colored samples in thin-layer chromatography are visualized by using ultraviolet light.

What is the purpose of the mobile phase in thin-layer chromatography?

• To carry the sample along the stationary phase

• To visualize the separated components

• To interact with the sample molecules

• To control the temperature of the process

Answer explanation

The purpose of the mobile phase in thin-layer chromatography is to carry the sample along the stationary phase, allowing for separation of components.

How does the Rf value change with an increase in the polarity of the solvent in thin-layer chromatography?

• Rf value decreases

• Rf value increases

• Rf value remains constant

• Rf value becomes negative

Answer explanation

The Rf value decreases with an increase in the polarity of the solvent in thin-layer chromatography due to stronger attraction between the sample and the stationary phase.

What is the stationary phase made of in thin-layer chromatography?

• Cellulose

• Silica gel

• Aluminum oxide

• Plastic polymer

Answer explanation

The stationary phase in thin-layer chromatography is made of silica gel, which is the correct choice.