QUALITY-TLC (READING MATERIALS)

What is the principle of thin layer chromatography (TLC)?

• Separation of compounds based on their weight

• Separation of compounds based on their odor

• Separation of compounds based on their color

• Separation of compounds based on differential migration in a stationary phase and mobile phase

Answer explanation

Thin layer chromatography (TLC) separates compounds based on their differential migration in a stationary phase and mobile phase.

What is the stationary phase in thin layer chromatography (TLC)?

• Thin layer of adsorbent material on a solid support

• Glass or plastic plate

• Solvent carrying the sample compounds

• UV light, iodine vapor, chemical reagents

Answer explanation

The stationary phase in thin layer chromatography (TLC) is a thin layer of adsorbent material on a solid support.

What is the mobile phase in thin layer chromatography (TLC)?

• Thin layer of adsorbent material on a solid support

• Solvent or solvent mixture carrying the sample compounds

• Separation of compounds based on their odor

• Ratio of compound's migration distance to solvent front's distance

Answer explanation

The mobile phase in thin layer chromatography (TLC) is the solvent or solvent mixture carrying the sample compounds.

How do compounds interact with the stationary phase in thin layer chromatography (TLC)?

• Based on their odor

• Based on their weight

• Based on polarity, size, charge, and other factors

• Based on their color

Answer explanation

Compounds interact with the stationary phase in TLC based on polarity, size, charge, and other factors, not based on their odor, weight, or color.

What is the visualization method for compounds in thin layer chromatography (TLC)?

• UV light, iodine vapor, chemical reagents

• Separation of compounds based on their color

• Thin layer of adsorbent material on a solid support

• Solvent or solvent mixture carrying the sample compounds

Answer explanation

The visualization method for compounds in thin layer chromatography (TLC) includes the use of UV light, iodine vapor, and chemical reagents.

What is the parameter used to measure compound's migration in thin layer chromatography (TLC)?

• Separation of compounds based on their odor

• Rf (retention factor): Ratio of compound's migration distance to solvent front's distance

• Solvent or solvent mixture carrying the sample compounds

• Thin layer of adsorbent material on a solid support

Answer explanation

The parameter used to measure compound's migration in thin layer chromatography (TLC) is the Rf (retention factor), which is the ratio of compound's migration distance to solvent front's distance.

What are the purposes of thin layer chromatography (TLC)?

• Analyzing complex mixtures, Identifying specific compounds, Monitoring reaction progress, Determining purity of substances

• Separation of compounds based on their odor

• Separation of compounds based on their color

• Separation of compounds based on their weight

Answer explanation

Thin layer chromatography (TLC) is used for analyzing complex mixtures, identifying specific compounds, monitoring reaction progress, and determining the purity of substances.

What are the advantages of thin layer chromatography (TLC)?

• Simple and cost-effective technique, Quick results, Requires minimal equipment

• Might not provide high resolution compared to HPLC, Less quantitative compared to other techniques

• Thin layer of adsorbent material on a solid support

• Solvent or solvent mixture carrying the sample compounds

Answer explanation

The advantages of thin layer chromatography (TLC) include being a simple and cost-effective technique, providing quick results, and requiring minimal equipment, which are all highlighted in the correct answer choice.

What are the limitations of thin layer chromatography (TLC)?

• Thin layer of adsorbent material on a solid support

• Solvent or solvent mixture carrying the sample compounds

• Simple and cost-effective technique, Quick results, Requires minimal equipment

• Might not provide high resolution compared to HPLC, Less quantitative compared to other techniques

Answer explanation

TLC might not provide high resolution compared to HPLC and is less quantitative compared to other techniques.

What is the separation time for compounds in thin layer chromatography (TLC)?

• Solvent or solvent mixture carrying the sample compounds

• Requires minimal equipment

• Thin layer of adsorbent material on a solid support

• Relatively quick, typically 15 to 30 minutes

Answer explanation

The separation time for compounds in thin layer chromatography (TLC) is relatively quick, typically 15 to 30 minutes.