
HPLC test
Authored by Kristaps Klavins
Science
University
Used 9+ times

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20 questions
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1.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
What happens to the given substance under reverse phase conditions when the pH of the mobile phase is raised?
Retention time doesn't change
Retention time will increase
Retention time will decrease
2.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
Which of the following mobile phases would provide the strongest elution conditions in reversed phase chromatography?
50% water, 50% acetonitrile
50% water, 25% acetonitrile, 25% THF
100% acetonitrile
50% acetonitrile, 50% THF
3.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
What will happen with the retention time of the given molecule in reversed-phase HPLC if the water content of the mobile phase is increased?
Increase
Decrease
Stay the same
4.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
Rank the solvents in order of increasing strength under HILIC conditions. Place the weakest solvent first and the strongest last.
Water – Methanol – Acetonitrile – Isopropanol
Water – Acetonitrile – Isopropanol – Methanol
Water – Methanol – Acetonitrile – Isopropanol
Acetonitrile – Isopropanol – Methanol – Water
5.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
What is the most commonly used stationary phase in reversed phase chromatography?
C18
Silicagel
C8
Carbon
6.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
In what order will these substances elute from the column in reverse-phase chromatography? Start with the least retained substance.
C A D B
A C D B
B A C D
C A B D
7.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
In what order will these substances elute from the column in reverse phase chromatography? Start with the least retained substance.
C A B D
B A C D
C B A D
A B D C
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