What is the advantage of using recombinant DNA technology in protein production?

Proteomics

Quiz
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Biology
•
Professional Development
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Easy
Mouesanao Kandjoze
Used 2+ times
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48 questions
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1.
MULTIPLE SELECT QUESTION
30 sec • 1 pt
precise manipulation of DNA sequences
expression of proteins in various host organisms
perfect recombinant DNA every time
Decreased antibiotic resistance
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of expressing a protein as a fusion construct containing an epitope tag?
to express the protein at high levels
to denature the protein
to purify the protein
to create an inter-membrane protein
3.
MULTIPLE SELECT QUESTION
45 sec • 1 pt
What type of affinity tags are commonly used in IMAC?
GFP
Glutathione S-transferase-tag
poly-adenine tails
His-tag
4.
OPEN ENDED QUESTION
10 sec • 1 pt
What does IMAC stand for?
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Answer explanation
Immobilised Metal Affinity Chromatography
5.
OPEN ENDED QUESTION
30 sec • 1 pt
What is the basic principle behind IMAC?
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Answer explanation
To selectively isolate a target protein from a complex mixture based on its specific interaction with an immobilized ligand. Affinity chromatography exploits the high affinity and specificity of interactions between the target protein and its ligand, allowing for highly efficient purification with minimal non-specific binding.
6.
OPEN ENDED QUESTION
30 sec • 1 pt
what is the significance/function of Nickel in the Ni-NTA resin?
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Answer explanation
Nickel binds to the His tag with high affinity via coordination bonds.
7.
OPEN ENDED QUESTION
3 mins • 1 pt
what is the difference between native and non-native protein purification?
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Answer explanation
Native: Aims to isolate the protein in its natural form, preserving its structural integrity and biological activity. Often employed for studying protein function, interactions with other molecules, and potential use in therapeutic applications. Milder purification techniques are crucial to minimize alterations to the protein's structure and post-translational modifications (PTMs) that might be crucial for its function.
Non-native: Focuses on isolating the protein in high yield and purity, often prioritizing quantity over maintaining the protein's native state.
Commonly used for protein characterization, structural studies, and large-scale production.
Harsher techniques might be employed, potentially affecting the protein's conformation and PTMs.
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