DNA Technology

Separation of DNA using electric current is called.....?

• Gel Electrophoresis
• Chromosome painting
• polymerage chain reaction
• Restrictive Enzyme Sorting 

What is the function of restriction enzymes?

• To add new nucleotides to the growing strand of DNA.
• To join the phosphodiester bonds of DNA fragments.

• To join the hydrogen bonds in DNA fragments

• To cut nucleic acids at specific sites.

The unpaired nucleotides produced by the action of restriction enzymes are referred to as

• sticky end.
• base sequence.
• single strands.
• restriction fragments.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

What enzyme forms phosphodiester bonds between DNA fragments

• DNA primase
• DNA helicase
• DNA ligase
• DNA polymerase

What is the most logical sequence of steps for transforming a bacteria.
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA and target DNA using the same restriction enzyme.
III) Extract plasmid DNA from bacterial cells.
IV) Combine the plasmid DNA and target DNA fragments.
V) Use DNA ligase to create phosphodiester bonds between the target DNA and plasmid

VI) isolate the target DNA or amplify the target DNA using PCR

• I, II, IV, III, V, VI

• III, VI, II, IV, V, I

• VI, II, III, V, IV, I

• III, VI, IV, V, I, II

Organisms that have DNA from a different species artificially inserted into their cells are called

• bioremediated
• transgenic
• cultured
• a clone

Tags

NGSS.HS-LS3-2

GMO stands for

• Genetically Made Organism
• Growing More Organisms
• Genetically Modified Organism
• Growing Many Organisms

All of the following are advantages of GMO's except

• pest resistant crops
• more nutritional foods
• Potential traits affecting natural selection

• creating more human insulin

You have benefited from selective breeding by having more agricultural produce than would have been possible otherwise.

• True
• False