Enzymology and automation 1st sessional Exam

Which of the following best defines an enzyme?

• A type of lipid molecule
• A type of protein molecule that speeds up chemical reactions
• A type of carbohydrate molecule
• A type of nucleic acid molecule

Enzymes are classified based on

• Their size
• Their speed of action
• Their substrate specificity and reaction type
• Their color

Isoenzymes are?

• Enzymes that have the same amino acid sequence
• Enzymes that catalyze the same reaction but have different amino acid sequences
• Enzymes that are only found in animals
• Enzymes that only work in acidic conditions

The concept of "lock and key" in enzyme-substrate interaction suggests?

• The enzyme changes its shape to fit the substrate perfectly
• The enzyme and substrate have complementary shapes
• The enzyme releases the substrate after catalysis
• The enzyme destroys the substrate during catalysis

The induced fit theory of enzyme-substrate interaction proposes that:

• Enzymes remain rigid and unchanged during catalysis
• Substrates force enzymes to undergo conformational changes
• Enzymes break down substrates into smaller molecules
• Enzymes do not require substrates to function

Activation energy is?

• The energy required to maintain enzyme activity
• The energy required to initiate a chemical reaction
• The energy released during a chemical reaction
• The energy stored in the enzyme-substrate complex

Binding energy in enzyme-substrate interactions refers to?

• The energy released when the enzyme binds to the substrate
• The energy required to break the enzyme-substrate complex
• The energy released when the enzyme-substrate complex forms
• The energy stored in the enzyme before substrate binding

Which of the following is NOT a factor affecting enzyme activity?

• pH
• Temperature
• Substrate concentration
• Enzyme color

Denaturation of enzymes can be caused by?

• High temperatures
• Low pH
• High substrate concentration
• All of the above

Competitive inhibitors is known as

• Bind to the active site of an enzyme
• Bind to a site other than the active site
• Increase the rate of enzyme catalysis
• Do not affect enzyme activity