
DNA Manipulation
Quiz
•
Biology
•
12th Grade
•
Practice Problem
•
Easy
Fiona Munro
Used 7+ times
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20 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the main purpose of using recombinant plasmids in genetic engineering?
To increase the size of the DNA molecule
To replicate viruses
To insert foreign DNA into a host cell
To remove unwanted DNA sequences
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which enzyme is used to seal the nicks in the backbone of DNA during the construction of recombinant DNA?
Restriction enzyme
DNA ligase
DNA polymerase
RNA polymerase
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
During gel electrophoresis, DNA fragments move through the gel towards the:
Negative pole, because DNA is negatively charged
Positive pole, because DNA is negatively charged
Negative pole, because DNA is positively charged
Positive pole, because DNA is positively charged
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the role of DNA polymerase in PCR (Polymerase Chain Reaction)?
To cut the DNA into smaller fragments
To seal the DNA fragments together
To synthesize new strands of DNA complementary to the template strands
To denature the double-stranded DNA
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following is NOT a step in bacterial transformation?
Incubation of the bacteria with recombinant DNA
Growth of bacteria on a nutrient agar plate
Lysis of the bacteria to release DNA
Selection of transformed bacteria
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The LacZ gene in a cloning vector is primarily used for:
Producing insulin
Cutting DNA at specific sites
Identifying transformed cells
Ligating DNA fragments
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following is a method used to produce insulin using recombinant DNA technology?
Inserting the human insulin gene into bacterial plasmids
Extracting insulin directly from human pancreas
Synthesizing insulin chemically in a laboratory
Extracting insulin from pig pancreas
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