MSc Sem2 Class 4

Select all correct facts about Zinc Finger Nuclease

• 30 amino acids, Zn+1 ion is present at the base, coordinated with either 4 Cys residues or 2 Met and 2 Arg, often multiple domains occur in a single DNA binding protein

• 20 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Cys and 2 His, often multiple domains occur in a single DNA binding protein

• 30 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Cys and 2 His, often multiple domains occur in a single DNA binding protein

• 30 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Arg and 2 His, often multiple domains occur in a single DNA binding protein

Explain histone acetylation and de-acetylation

• HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to arginine residues in the core histones like H3 and H4

  HDAC - Histone deacetylase, removes acetyl group

• HAT - Histone Acetyl Transferase, removes acetyl group from Acetyl-coA to lysine residues in the core histones like H3 and H4

  HDAC - Histone deacetylase, removes acetyl group

• HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to lysine residues in the core histones like H2A and H4

  HDAC - Histone deacetylase, removes acetyl group

• HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to lysine residues in the core histones like H3 and H4

  HDAC - Histone deacetylase, removes acetyl group

Explain Histone acetylation and de-acetylation

• Acetylation - Makes DNA more accessible, allows transcription to take place

  De-acetylation - Makes DNA accessible, stops transcription from taking place

• Acetylation - Makes DNA more inaccessible, allows transcription to take place

  De-acetylation - Makes DNA inaccessible, stops transcription from taking place

• Acetylation - Makes DNA more accessible, allows transcription to take place

  De-acetylation - Makes DNA inaccessible, stops transcription from taking place

• Acetylation - Makes RNA more accessible, allows transcription to take place

  De-acetylation - Makes RNA inaccessible, stops transcription from taking place

Select the correct fact about HATs

• There are two types A and B, type A HATs are present in the cytoplasm and have a bromodomain, they acetylate core histones

  type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm

• There are two types A and B, type A HATs are present in the nucleus and have a bromodomain, they acetylate core histones

  type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm

• There are two types A and B, type A HATs are present in the nucleus and lack a bromodomain, they acetylate core histones

  type B HATs are with bromodomain and acetylate newly synthesized histones in the cytoplasm

• There are two types A and B, type A HATs are present in the nucleus and have a bromodomain, they deacetylate core histones

  type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm

What do you know about nucleosome sliding?

• Is a mechanism of chromatin remodeling, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from GTP hydrolyzing.

• Is a mechanism of chromatin remodeling, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.

• Is a mechanism of chromatin condensation, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.

• Is a mechanism of chromatin remodeling, proteasomes are found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.

SWI/SNF ________________________

• Was first discovered in C.elegans and was found to regulate function of HO gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase

• Was first discovered in yeast and was found to regulate function of SUC2 gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase

• Was first discovered in yeast and was found to regulate function of HO gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase

• Was first discovered in yeast and was found to regulate function of HO gene involved in mating type switching and also in regulation of pik53 gene that encodes invertase

Explain euchromatin and heterochromatin

• Euchromatin - Transcriptionally inactive, more condensed.

  Heterochromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes

• Euchromatin - Transcriptionally active, more condensed.

  Heterochromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes

• Euchromatin - Transcriptionally inactive, more condensed.

  Heterochromatin - Transcriptionally inactive region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes

• Euchromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes

  Heterochromatin - Transcriptionally inactive, more condensed.

Select all correct correct facts about nucleosome eviction

• All members of the SWI/SNF family and only a subset of ISWI remodelers are unable to eject histone dimers.

• All members of the SWI/SNF family and all ISWI remodelers are able to eject histone dimers.

• All members of the SWI/SNF family and only a subset of ISWI remodelers are able to eject histone dimers.

• All members of the SWI/SNF family and only a subset of ISWI remodelers are able to slide on histone dimer

Select all correct facts about histone exchange

• The SWR1 complex catalyzes the exchange of nucleosomal H2A for the H2B variant.

  Similarly, H3 can be exchanged by H3.3.

• The SWR1 complex catalyzes the exchange of nucleosomal H2A for the H2AZ variant.

  Similarly, H3 can be exchanged by H3.3.

• The ISWI complex catalyzes the exchange of nucleosomal H2A for the H2AZ variant.

  Similarly, H3 can be exchanged by H3.3.

• The SWR1 complex catalyzes the eviction of nucleosomal H2A for the H2AZ variant.

  Similarly, H3 can be exchanged by H3.3.

Pick the correct role of transferrin and ferritin

• Ferritin is responsible for metabolizing iron, while transferrin binds to iron and transports it in the blood

• Ferritin is responsible for storing iron in a non-toxic form, while transferrin binds to iron and transports it in the blood

• Ferritin binds to iron and transports it in the blood while transferrin is responsible for storing iron in a non-toxic form

• Ferritin is responsible for storing iron in a non-toxic form, while transferrin binds to iron and transports it outside the cell