Chapter 10

To artificially produce genetically identical individuals

To obtain a copy of DNA (gene) and replicate this DNA (gene) in host cells

To artificially modify genes (DNA) and then transform a host with it

The Restriction Nucleus is cu into

The Separation of DNA fragments

Reorder the following

• Obtain a pure DNA fragment

• Recombine DNA

• .Transformation to Organism

enzymes that cut DNA at specific sites

same restriction enzyme will cut the same cutting site and generate complementary ends at different DNA molecules for recombining

• True

• False

Which enzyme is used to seal the gaps between the fragments of DNA in recombinant DNA technology?

• Ligase

• Helicase

• Primase

• Topoisomerase

What is the role of DNA polymerase in PCR?

• To unwind the DNA double helix

• To cut the DNA at specific sites

• To add nucleotides to the 3' end of the DNA strand

• To join Okazaki fragments