Викторина по теме Southern Blotting

What is the technique of Southern Blotting?

• Method for detecting specific DNA sequences in a complex mixture

• Method for detecting specific mutations in DNA

• Method for detecting specific proteins in cells

• Method for detecting specific genes in an organism

What steps are involved in the Southern Blotting process?

• Study of protein structure, gene comparison, mutation analysis, DNA cloning

• Cutting DNA into fragments, separating them by size, transferring to filter, exposing to radioactive probe

• Genome sequencing, DNA amplification, electrophoresis, hybridization

• Metabolism study, biochemical reaction analysis, protein synthesis, cell division

How is the visualization of a specific DNA sequence done using Southern Blotting?

• By irradiating the filter with radioactive DNA probe

• By staining the filter with a special dye

• By adding enzymes to the filter

• By heating the filter to a high temperature

What does gel electrophoresis represent in the context of the Southern Blotting method?

• Method of genome sequencing

• Gene cloning method

• DNA amplification method

• Method of separating DNA fragments by size and charge

How is the transfer of DNA fragments to the filter done in the Southern Blotting method?

• By transferring fragments from the gel to the filter

• By staining the filter with a special dye

• By adding enzymes to the filter

• By exposing the filter to a radioactive DNA probe

What does a radioactive DNA probe represent in the Southern Blotting method?

• Enzyme used for DNA cloning

• Enzyme used for DNA cutting

• Complementary sequence to the gene of interest

• Dye for staining the filter

How is the radioactive DNA probe visualized on the filter in the Southern Blotting method?

• How is the radioactive DNA probe visualized on the filter in the Southern Blotting method?

• By exposing the filter to X-rays

• By staining the filter with a special dye

• By adding enzymes to the filter

• By heating the filter to a high temperature

What enzymes are used to cut DNA in the Southern Blotting method?

• Restriction enzymes

• Replication enzymes

• DNA synthesis enzymes

• Transcription enzymes

What happens after DNA fragments have been cut by restriction enzymes in the Southern Blotting method?

• Fragments can be removed from the reaction mixture

• Fragments can be stained with a special dye

• Fragments can be combined into one large fragment

• Fragments can be separated into single-stranded fragments

How does hybridization of radioactive DNA probe with the genome of interest occur in the Southern Blotting method?

• The probe anneals to the gene of interest on the filter

• The probe anneals to the gene of interest in the cell

• The probe anneals to the gene of interest on the gel

• The probe anneals to the gene of interest in the reaction mixture