Understanding CRISPR and Genetics

How many genes approximately does the human genome contain?

• Over 20,000 genes

• About 10,000 genes

• More than 50,000 genes

• Around 5,000 genes

Tags

NGSS.HS-LS3-2

What is the basic structure of DNA?

• Single helix

• Triple helix

• Quadruple helix

• Double helix

Which base pair matches are correct in DNA?

• A with G, T with C

• A with C, G with T

• A with T, G with C

• G with A, C with T

What has DNA sequencing helped identify?

• Genes affecting disease risk

• Viral genomes

• Cellular functions

• Protein structures

What is CRISPR based on?

• A viral replication process

• A bacterial defense system

• A human immune response

• An animal's genetic mutation

What role does Cas9 play in CRISPR?

• Transcribes genes

• Modifies proteins

• Cuts DNA

• Replicates RNA

How does CRISPR-Cas9 find its target DNA sequence?

• Using a guide protein

• Through a matching RNA sequence

• Via a DNA template

• By a randomly generated code

What is the role of guide RNA in the CRISPR system?

• It cuts the DNA

• It transcribes the new gene

• It repairs the DNA

• It identifies the target DNA sequence

What happens when Cas9 cuts the DNA?

• The cell repairs the cut, often with errors

• Nothing, the cut is superficial

• The DNA is permanently damaged

• The gene is replicated

Why is the repair process after Cas9 cuts the DNA error-prone?

• Because the repair mechanisms can introduce mutations

• Due to the complexity of the DNA structure

• Because the cell lacks the necessary enzymes

• Because CRISPR interferes with the repair