Biotechnology

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

What is the role of the guide RNA?

• It helps the cell repair the DNA

• ensures that the Cas9 enzyme cuts at the right point in the genome

• Recognizes PAM sequences and opens up the DNA

• makes a cut across both strands of the DNA

How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes

The following sequence is a known GENE MUTATION that geneticists would like to remove. What would be the guide RNA they need to build into a CRISPR-Cas9 enzyme?

TTA CCG

• AAU GGC

• AAT GGC

• TTA CCG

• GCC ATT

What does the cas9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

CRISPR involves "gene editing". Dr. Doudna distinguishes "gene editing" from "genetic modification" like we have been doing prior to CRISPR. Which of the following is a big difference between "gene editing" and "genetic modification"?

• Gene editing can move DNA from one organsim to another

• Genetic modification can move DNA from one organism to another

• Gene editing can only change A's to C's

• Gene editing can move DNA from one organism to another

What is the primary purpose of gene editing ?

• To create decorative plants

• To increase the size of fruits and vegetables

• To study the history of DNA

• To treat diseases and improve agriculture

Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.

• building

• editing

• destroying

• synthesizing

What is PAM?

• a 3 letter sequence for binding on the target DNA

• the main gene portion of the DNA

• the cut site for Cas9

• a 10 letter sequence for binding on the target DNA

Which of the following is an example of biotech in which researchers sequenced and mapped ALL genes in the human species?

• Stem Cells

• Human Genome Project

• Cloning

• Gene Therapy